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A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents

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journal contribution
posted on 2024-06-18, 04:49 authored by D Premilovac, RJ Gasperini, S Sawyer, A West, Michelle KeskeMichelle Keske, BV Taylor, L Foa
Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80-200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans.

History

Journal

Scientific Reports

Volume

7

Article number

ARTN 14158

Pagination

1 - 10

Location

England

Open access

  • Yes

ISSN

2045-2322

eISSN

2045-2322

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2017, The Authors

Issue

1

Publisher

NATURE PUBLISHING GROUP