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A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC
journal contribution
posted on 2015-01-01, 00:00 authored by Danielle Bassanese, A Soliven, Xavier ConlanXavier Conlan, R A Shalliker, Neil BarnettNeil Barnett, Paul StevensonThis paper describes a non-destructive "peak parking" protocol in order to assess the axial heterogeneity of an in situ modified monolithic column for high performance liquid chromatography; a "gradient stationary phase" was designed whereby the ligand density decreases along the length of the rod in the "forward flow" configuration. The results of multi-location peak parking demonstrated a consistent increase in peak variance from the 1 cm position of the column to the 9 cm location. This increase in band broadening supported the theory of a decreasing ligand density along the length of this gradient column. This is consistent with efficiency measurements performed in both the forward and reverse flow directions, with an improved efficiency (15% increase in N m-1) in the reverse direction. These results are consistent with theoretical investigations into stationary phase gradients.
History
Journal
Analytical methods: advancing methods and applicationsVolume
7Issue
17Pagination
7177 - 7185Publisher
Royal Society of ChemistryLocation
Cambridge, Eng.Publisher DOI
ISSN
1759-9660eISSN
1759-9679Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, Royal Society of ChemistryUsage metrics
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No categories selectedKeywords
Science & TechnologyPhysical SciencesLife Sciences & BiomedicineTechnologyChemistry, AnalyticalFood Science & TechnologySpectroscopyChemistryPHASE LIQUID-CHROMATOGRAPHYGASEOUS-DIFFUSION COEFFICIENTSPARKING-MOMENT ANALYSISSTATIONARY-PHASEMASS-TRANSFERPEAK-PARKINGRETENTION CHARACTERISTICSENANTIOMER SEPARATIONSLONGITUDINAL DIFFUSIONSURFACE COVERAGE
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