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A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction

journal contribution
posted on 2001-07-27, 00:00 authored by Cenk SuphiogluCenk Suphioglu, G Schäppi, J Kenrick, D Levy, J M Davies, R E O'Hehir
The aim of this study was to investigate the molecular basis of human IgE-allergen interaction by screening a phage-displayed peptide library with an allergen-specific human IgE-mimicking monoclonal antibody (mAb). A mAb that reacted with major grass pollen allergens was successfully identified and shown to inhibit human IgE-allergen interaction. Biopanning of a phage-displayed random peptide library with this mAb yielded a 12 amino acid long mimotope. A synthetic peptide based on this 12-mer mimotope inhibited mAb and human IgE binding to grass pollen extracts. Our results indicate that such synthetic peptide mimotopes of allergens have potential as novel therapeutic agents.

History

Journal

FEBS letters

Volume

502

Issue

1-2

Pagination

46 - 52

Publisher

John Wiley & Sons

Location

Chichester, Eng.

ISSN

0014-5793

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2015, Federation of European Biochemical Societies

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