A novel in vitro whole plant system for analysis of polyphenolics and their antioxidant potential in cultivars of Ocimum basilicum
journal contributionposted on 2014-01-01, 00:00 authored by Shivani Srivastava, David Cahill, Xavier ConlanXavier Conlan, Alok AdholeyaAlok Adholeya
Plants are an important source for medicinal compounds. Chemical screening and selection is critical for identification of compounds of interest. Ocimum basilicum (Basil) is a rich source of polyphenolics and exhibits high diversity, therefore bioprospecting of a suitable cultivar is a necessity. This study reports on the development of a true to type novel "in vitro system" and its comparison with a conventional system for screening and selection of cultivars for high total phenolics, individual polyphenolics, and antioxidant content. We have shown for the first time using online acidic potassium permanganate chemiluminescence that extracts from Ocimum basilicum showed antioxidant potential. The current study identified the cultivar specific composition of polyphenolics and their antioxidant properties. Further, a distinct relationship between plant morphotype and polyphenolic content was also found. Of the 15 cultivars examined, "Holy Green", "Red Rubin", and "Basil Genovese" were identified as high polyphenolic producing cultivars while "Subja" was determined to be a low producer. The "in vitro system" enabled differentiation of the cultivars in their morphology, polyphenolic content, and antioxidant activity and is a cheap and efficient method for bioprospecting studies.
JournalJournal of agricultural and food chemistry
Pagination10064 - 10075
Publication classificationC Journal article; C1 Refereed article in a scholarly journal
Copyright notice2014, ACS Publications
Ocimum basilicumantioxidantbasilbioprospectingchemiluminescencechicoric acidrosmarinic acidScience & TechnologyLife Sciences & BiomedicinePhysical SciencesAgriculture, MultidisciplinaryChemistry, AppliedFood Science & TechnologyAgricultureChemistryACIDIC POTASSIUM-PERMANGANATECHEMICAL-COMPOSITIONPHENOLIC COMPOSITIONSWEET BASILL.ASSAYSIDENTIFICATIONOFFICINALIS