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A novel sensing strategy for DNA analysis using nanoscale graphene oxide-coated microbeads

Version 2 2024-06-18, 13:44
Version 1 2019-03-07, 09:39
journal contribution
posted on 2024-06-18, 13:44 authored by Tuong Ngoc-Gia Nguyen, Changyoon Baek, Jaekwang Kim, Songhun Yoon, Junhong Min
Graphene oxide (GO) is a powerful material for the detection of various biological targets including nucleic acids, proteins, and metal ions. Herein, we introduce a rapid, sensitive, and selective tool for pathogen detection by combining bead beating-based DNA extraction and graphene oxide-based fluorescence quenching. This combination was achieved by the fabrication of fluorescent dye-tagged probe/GO/glass beads. The target nucleic acid sequence was extracted from Staphylococcus aureus (S. aureus) using a bead beating tool and GO-coated glass beads (GO beads) on which an FITC-tagged probe was adsorbed. The interaction between single strand DNA (ss-DNA) and GO was disrupted by the formation of double strand DNA (ds-DNA), resulting in stronger fluorescence emission in ds-DNA-GO mixture compared with ss-DNA-GO mixture. Therefore, when the target DNA hybridized with the FITC-tagged probes, the probes detached from the GO beads and their fluorescence was renewed. This method allows detection of Staphylococcus aureus at a concentration as low as 105 CFU/ml without sample or signal amplification.

History

Journal

Journal of nanoscience and nanotechnology

Volume

17

Pagination

7986-7990

Location

Valencia, Calif.

ISSN

1533-4880

eISSN

1533-4899

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2017, American Scientific Publishers

Issue

11

Publisher

American Scientific Publishers