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A quick colorimetric method for total lipid quantification in microalgae

Version 2 2024-06-06, 05:59
Version 1 2016-04-08, 12:54
journal contribution
posted on 2024-06-06, 05:59 authored by AR Byreddy, A Gupta, Colin BarrowColin Barrow, Munish Puri
Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulpho-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method was successfully tested on marine thraustochytrid strains and vegetable oils. The colorimetric method results correlated well with gravimetric method estimates. The new method was less time consuming than gravimetric analysis and is quantitative for lipid determination, even in the presence of carbohydrates, proteins and glycerol.

History

Journal

Journal of Microbiological Methods

Volume

125

Pagination

28-32

Location

Netherlands

ISSN

0167-7012

eISSN

1872-8359

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2016, Elsevier

Publisher

ELSEVIER SCIENCE BV