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A reservoir of brown adipocyte progenitors in human skeletal muscle

journal contribution
posted on 01.09.2008, 00:00 authored by M Crisan, L Casteilla, L Lehr, M Carmona, A Paolini-Giacobino, S Yap, B Sun, B Léger, A Logar, L Penicaud, P Schrauwen, David Cameron-Smith, Aaron RussellAaron Russell, B Peault, J P Giacobino
Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-{gamma} (PPAR{gamma}) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPAR{gamma} agonist treatment. All the studies concerning UCP1 expression in adult humans have until now been focused on the white adipose tissue. Here we show for the first time the existence in human skeletal muscle and the prospective isolation of progenitor cells with a high potential for UCP1 expression. The discovery of this reservoir generates a new hope of treating obesity by acting on energy dissipation.

History

Journal

Stem cells : the international journal of cell differentiation and proliferation

Volume

26

Issue

9

Pagination

2425 - 2433

Publisher

AlphaMed Press

Location

Dayton, Oh.

ISSN

1066-5099

eISSN

1549-4918

Language

eng

Publication classification

C1 Refereed article in a scholarly journal; C Journal article

Copyright notice

2008, AlphaMed Press