dichtl-roleforssu72-2002.pdf (644.94 kB)
A role for SSU72 in balancing RNA polymerase II transcription elongation and termination
journal contribution
posted on 2002-11-01, 00:00 authored by Bernhard DichtlBernhard Dichtl, D Blank, M Ohnacker, A Friedlein, D Roeder, H Langen, W KellerInteractions of pre-mRNA 3′end factors and the CTD of RNA polymerase II (RNAP II) are required for transcription termination and 3′end processing. Here, we demonstrate that Ssu72p is stably associated with yeast cleavage and polyadenylation factor CPF and provide evidence that it bridges the CPF subunits Pta1p and Ydh1p/Cft2p, the general transcription factor TFIIB, and RNAP II via Rpb2p. Analyses of ssu72-2 mutant cells in the absence and presence of the nuclear exosome component Rrp6p revealed defects in RNAP II transcription elongation and termination. 6-azauracil, that reduces transcription elongation rates, suppressed the ssu72-2 growth defect at 33°C. The sum of our analyses suggests a negative influence of Ssu72p on RNAP II during transcription that affects the commitment to either elongation or termination.
History
Journal
Molecular cellVolume
10Issue
5Pagination
1139 - 1150Publisher
Cell PressLocation
Cambridge, Mass.Publisher DOI
ISSN
1097-2765eISSN
1097-4164Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2002, ElsevierUsage metrics
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