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A simple device for rapid quantification of cell number from equine buccal swab samples

Version 2 2024-06-04, 04:57
Version 1 2018-03-16, 16:53
journal contribution
posted on 2018-02-26, 00:00 authored by Kim Quayle, Egan DoevenEgan Doeven, Richard AlexanderRichard Alexander, Giorgio De GuzmanGiorgio De Guzman, A F Clarke, Paul FrancisPaul Francis, Xavier ConlanXavier Conlan, Stephen Haswell
Biological samples are notoriously difficult to use within portable miniaturised devices, often due to complex sample matrices or variation between cellular samples. Herein we describe a device that utilises a correction based upon the total soluble cellular protein and absorbance measurements for the accurate quantification of cell number from equine buccal swabs. Hemocytometer cell counts were initially conducted to determine average mammalian and bacterial contributions (97.66% and 2.34% respectively). Secondly, cells were lysed and centrifuged before the supernatant was utilised within a Bradford's ratio metric absorbance assay using a microplate reader. A correlation coefficient of r ¼ 0.992 was determined, indicating this method was successful in utilising total soluble cellular protein for the quantification of cell number in unknown
biological samples of this type. Upon the identification of a strong linear correlation coefficient, a portable microfluidic device was developed (r ¼ 0.983) as a highly specific platform for real time buccal cell number quantification with the ability to be used for sample correction for buccal swab samples as low as 7.28 mg mL1 in economic downstream biological diagnostic devices

History

Journal

Analytical methods: advancing methods and applications

Volume

10

Issue

13

Pagination

1523 - 1528

Publisher

Royal Society of Chemistry

Location

London, Eng.

ISSN

1759-9660

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2018, The Royal Society of Chemistry

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