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A whole body, in vivo, fatty Aacid balance method to quantify PUFA metabolism (desaturation, elongation and beta-oxidation)
journal contribution
posted on 2007-11-01, 00:00 authored by Giovanni TurchiniGiovanni Turchini, David FrancisDavid Francis, S De SilvaCurrently there are several contrasting methods utilized for estimating elongation and desaturation of fatty acids and their general metabolism. The majority of these methods involve an ex vivo approach, requiring expensive and sophisticated equipment, likely to result in considerable variation in enzyme activity between and within species. In the present paper we introduce a further development of the whole-body fatty acid balance method for the estimation of the elongation and desaturation of fatty acids. This method though receiving considerable attention because of its simplicity and reliability has yet to be presented in detail. Theoretically, the whole-body fatty acid balance method can potentially be applied to any organism and requires little more than a gas chromatography unit for fatty acid analysis and elementary calculations. As such in this paper we attempt to spell out in detail the theoretical basis and the methods of application drawing specific examples. Using the present method it is possible to measure the fate of individual fatty acids towards desaturation, elongation and oxidation and calculate the elongase, Δ-6 desaturase and Δ-5 desaturase activities.
History
Journal
LipidsVolume
42Issue
11Pagination
1065 - 1071Publisher
SpringerLocation
Berlin , GermanyPublisher DOI
ISSN
0024-4201eISSN
1558-9307Language
engNotes
Springer online: Wednesday, August 15, 2007Publication classification
C1 Refereed article in a scholarly journalCopyright notice
2007, SpringerUsage metrics
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