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Accelerated growth of preosteoblastic cells on ultrafine grained titanium

journal contribution
posted on 2009-09-15, 00:00 authored by Y Estrin, C Kasper, S Diederichs, Rimma LapovokRimma Lapovok
This work is part of a general effort to demonstrate the effect of the bulk microstructure of titanium as a model bone implant material on viability of osteoblasts (bone-forming cells). The objective of this work was to study the proliferation of preosteoblastic MC3T3-E1 cells extracted from mice embryos on commercial purity titanium substrates. Two distinct states of titanium were considered: as-received material with an average grain size of 4.5 microm and that processed by equal channel angular pressing (ECAP), with an average grain size of 200 nm. We report the first results of an in vitro study into the effect of this extreme grain refinement on viability and proliferation of MC3T3-E1 cells. By means of MTT assays it was demonstrated that ECAP processing of titanium enhances MC3T3-E1 culture proliferation in a spectacular way. This finding suggests that bone implants made from ECAP processed titanium may promote bone tissue growth.

History

Journal

Journal of biomedical materials research : part a

Volume

90A

Pagination

1239-1242

Location

Chichester, Eng.

eISSN

1552-4965

Language

eng

Publication classification

C Journal article, C1.1 Refereed article in a scholarly journal

Copyright notice

2008, Wiley Periodicals

Issue

4

Publisher

Wiley