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Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers
journal contribution
posted on 2009-02-25, 00:00 authored by Y Yin, M Bilek, D McKenzie, N Nosworthy, A Kondyurin, H Youssef, M Byrom, Wenrong YangWenrong YangSmooth polymerized surfaces, suitable for biochemical and biomedical applications, were deposited using a modified plasma enhanced chemical vapour deposition method with acetylene as a reaction precursor. Horseradish peroxidase (HRP) activity assays showed that the protein immobilized on the plasma polymerized surfaces maintained its biological function for a much longer period of time compared to that on uncoated surfaces. The kinetics of HRP attachment to the plasma polymerized surfaces were analyzed using quartz crystal microbalance with dissipation analysis. Spectroscopic ellipsometry and attenuated total reflection Fourier transform infrared spectroscopy were used to determine the thickness and the quantity of the attached protein. The results showed that the plasma polymerized surfaces provided a high density of attachment sites to covalently immobilize a dense monolayer of proteins.
History
Journal
Surface and coatings technologyVolume
203Issue
10-11Pagination
1310 - 1316Publisher
Elsevier B. V.Location
Amsterdam, The NetherlandsPublisher DOI
ISSN
0257-8972Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2008, Elsevier B.V.Usage metrics
Keywords
plasma polymerizationprotein immobilizationcovalent attachmentquartz crystal microbalanceFTIRellipsometerScience & TechnologyTechnologyPhysical SciencesMaterials Science, Coatings & FilmsPhysics, AppliedMaterials SciencePhysicsSELF-ASSEMBLED MONOLAYERSBIOMEDICAL APPLICATIONSBINDINGFILMSCondensed Matter Physics
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