File(s) under permanent embargo
Activation of the selenopritein SEPS1 gene expression by pro-inflammatory cytokines in Hep G2 cells
journal contribution
posted on 2006-03-07, 00:00 authored by Yuan Gao, N Hannan, Stephen Wanyonyi, Nicky Konstantopoulos, J Pagnon, Helen Feng, Jeremy Jowett, K H Kim, Ken WalderKen Walder, Gregory CollierSEPS1 (also called selenoprotein S, SelS) plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. In this report, we have identified two binding sites for the nuclear factor kappa B in the human SEPS1 promoter. SEPS1 gene expression, protein levels and promoter activity were all increased 2–3-fold by TNF-α and IL-1β in HepG2 cells. We have also confirmed that the previously proposed ER stress response element GGATTTCTCCCCCGCCACG in the SEPS1 proximate promoter is fully functional and responsive to ER stress. However, concurrent treatment of HepG2 cells with IL-1β and ER stress produced no additive effect on SEPS1 gene expression. We conclude that SEPS1 is a new target gene of NF-κB. Together with our previous findings that SEPS1 may regulate cytokine production in macrophage cells, we propose a regulatory loop between cytokines and SEPS1 that plays a key role in control of the inflammatory response.
History
Journal
CytokineVolume
33Issue
5Pagination
246 - 251Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
1043-4666eISSN
1096-0023Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2006, Elsevier LtdUsage metrics
Keywords
selenoproteinglucose-regulated proteinER stresscytokinegene expressionScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyCell BiologyImmunologyENDOPLASMIC-RETICULUMKAPPA-BTRANSCRIPTION FACTORRETRO-TRANSLOCATIONLIVER-CELLSPROTEINSPROMOTERCYTOSOLSTRESSTARGETGeneticsImmunology
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC