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Acute HIIE elicits similar changes in human skeletal muscle mitochondrial H₂O₂ release, respiration, and cell signaling as endurance exercise even with less work

journal contribution
posted on 2018-11-01, 00:00 authored by Adam TrewinAdam Trewin, Lewan ParkerLewan Parker, Chris ShawChris Shaw, Danielle HiamDanielle Hiam, Andrew GarnhamAndrew Garnham, Itamar Levinger, Glenn K McConell, Nigel K Stepto
It remains unclear whether high-intensity interval exercise (HIIE) elicits distinct molecular responses to traditional endurance exercise relative to the total work performed. We aimed to investigate the influence of exercise intensity on acute perturbations to skeletal muscle mitochondrial function (respiration and reactive oxygen species), metabolic and redox signaling responses. In a randomized, repeated measures crossover design, eight recreationally active individuals (24 ± 5 years; VO2peak 48 ± 11 mL.kg-1.min-1) undertook continuous moderate-intensity (CMIE: 30 min, 50% peak power output [PPO]), high-intensity interval (HIIE: 5x4 min, 75% PPO, work-matched to CMIE), and low-volume sprint interval (SIE: 4x30 s) exercise, ≥7 days apart. Each session included muscle biopsies at baseline, immediately and 3 h post-exercise for high-resolution mitochondrial respirometry ( JO2) and H2O2 emission ( JH2O2), gene and protein expression analysis. Immediately post-exercise and irrespective of protocol, JO2 increased during complex I+II leak/state-4 respiration but JH2O2 decreased (p<0.05). AMP-activated protein kinase (AMPK) and acetyl co-A carboxylase (ACC) phosphorylation increased ~1.5 and 2.5-fold respectively, while thioredoxin-reductase-1 protein abundance was ~35% lower after CMIE vs. SIE (p<0.05). At 3 hours post-exercise, regardless of protocol, JO2 was lower during both ADP-stimulated state-3 OXPHOS and uncoupled respiration (p<0.05) but JH2O2 trended higher (p<0.08); PPARGC1A mRNA increased ~13-fold, and peroxiredoxin-1 protein decreased ~35%. In conclusion, intermittent exercise performed at high intensities has similar dynamic effects on muscle mitochondrial function compared with endurance exercise, irrespective of whether total workload is matched. This suggests exercise prescription can accommodate individual preferences while generating comparable molecular signals known to promote beneficial metabolic adaptations.

History

Journal

American journal of physiology: regulatory, integrative and comparative physiology

Volume

315

Issue

5

Pagination

R1003 - R1016

Publisher

American Physiological Society

Location

Bethesda, Md.

ISSN

0363-6119

eISSN

1522-1490

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, American Physiological Society