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Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

journal contribution
posted on 2014-01-01, 00:00 authored by P Pahuja, A Srinivasan, Munish Puri
X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.

History

Journal

Molecular and cellular biochemistry

Volume

389

Issue

1-2

Pagination

133 - 139

Publisher

Springer

Location

Berlin, Germany

ISSN

0300-8177

eISSN

1573-4919

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2014, Springer

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