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Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle

journal contribution
posted on 2009-05-01, 00:00 authored by Chris ShawChris Shaw, M Sherlock, P M Stewart, A J M Wagenmakers
Intramyocellular lipids (IMCL) are stored as discrete lipid droplets which are associated with a number of proteins. The lipid droplet-associated protein adipophilin (the human orthologue of adipose differentiation-related protein) is ubiquitously expressed and is one of the predominant lipid droplet-proteins in skeletal muscle. The aim of this study was to investigate the subcellular distribution of adipophilin in human muscle fibres and to measure the colocalization of adipophilin with IMCL. Muscle biopsies from six lean male cyclists (BMI 23.4 +/- 0.4, aged 31 +/- 2 years, W (max) 346 +/- 8) were stained for myosin heavy chain type 1, IMCL, adipophilin and mitochondria using immunofluorescence and viewed with widefield and confocal fluorescence microscopy. The present study shows that like IMCL, the adipophilin content is ~twofold greater in type I skeletal muscle fibres and is situated in the areas between the mitochondrial network. Colocalization analysis demonstrated that 61 +/- 2% of IMCL contain adipophilin. Although the majority of adipophilin is contained within IMCL, 36 +/- 4% of adipophilin is not associated with IMCL. In conclusion, this study indicates that the IMCL pool is heterogeneous, as the majority but not all IMCL contain adipophilin.

History

Journal

Histochemistry and cell biology

Volume

131

Issue

5

Pagination

575 - 581

Publisher

Springer

Location

Heidelberg, Germany

ISSN

0948-6143

eISSN

1432-119X

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2009, Springer