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Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(Dimethylsiloxane) (PDMS) polymers
journal contribution
posted on 2016-06-01, 00:00 authored by H Halimoon, A R Hussain, Abbas KouzaniAbbas Kouzani, M N H Z AlamIn this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated from poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) polymers. Cell density was measured online whilst agitation rates and the temperature of the reactor content can be tightly controlled to desired set-point values. As proof-of-concept, various S. cerevisae fermentation experiments were conducted. In every experiment, the minibioreactor operated stably for the entire length of operation which was nearly 40 h with very minimal volume loss i.e. about 2.8 μ·h-1 at 37°C. The minibioreactor has the maximum oxygen transfer rate (OTR) of 16.6 mmol·L-1·h-1 under the agitation rate of 300 rpm. Under these conditions, cell specific growth rate as high as 0.291 h-1 was obtained. The experimental data in the minibioreactor operation was also reproducible using shake flask where similar growth profiles were attained under a similar growth conditions.
History
Journal
Sains MalaysianaVolume
45Issue
6Pagination
969 - 976Publisher
National University of Malaysia PressLocation
[Kuala Lumpur, Malaysia]ISSN
0126-6039Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2016, National University of Malaysia PressUsage metrics
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