selig-aktas160metabolic-2010.pdf (202.65 kB)
Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women
journal contribution
posted on 2010-01-01, 00:00 authored by I Levinger, Kirsten HowlettKirsten Howlett, J Peake, Andrew GarnhamAndrew Garnham, D Hare, G Jerums, Steve SeligSteve Selig, C GoodmanThis study investigated the association between the basal (rest) insulin-signaling proteins, Akt, and the Akt substrate AS160, metabolic risk factors, inflammatory markers and aerobic fitness, in middle-aged women with varying numbers of metabolic risk factors for type 2 diabetes. Methods: Sixteen women (n=16) aged 51.3±5.1 (mean ±SD) years provided muscle biopsies and blood samples at rest. In addition, anthropometric characteristics and aerobic power were assessed and the number of metabolic risk factors for each participant was determined (IDF criteria). Results: The mean number of metabolic risk factors was 1.6±1.2. Total Akt was negatively correlated with IL-1β (r = -0.45, p = 0.046), IL-6 (r = -0.44, p = 0.052) and TNF-α (r = -0.51, p = 0.025). Phosphorylated AS160 was positively correlated with HDL (r = 0.58, p= 0.024) and aerobic fitness (r = 0.51, p=0.047). Furthermore, a multiple regression analysis revealed that both HDL (t=2.5, p=0.032) and VO2peak (t=2.4, p=0.037) were better predictor for phosphorylated AS160 than TNF-α or IL-6 (p>0.05). Conclusions: Elevated inflammatory markers and increased metabolic risk factors may inhibit insulin-signaling protein phosphorylation in middle-aged women, thereby increasing insulin resistance under basal conditions. Furthermore, higher HDL and fitness levels are associated with an increase AS160 phosphorylation, which may in turn reduce insulin resistance.
History
Journal
Exercise immunology reviewVolume
16Pagination
98 - 104Publisher
Human KineticsLocation
Champaign, Ill.ISSN
1077-5552Language
engNotes
Reproduced with the specific permission of the copyright owner.Publication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2010, Human KineticsUsage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC