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Alpha-Lactalbumin and lactose concentrations in rat milk during lactation.

Version 2 2024-06-13, 14:58
Version 1 2021-11-22, 08:40
journal contribution
posted on 2024-06-13, 14:58 authored by KR Nicholas, PE Hartmann, BL McDonald
Homogeneous rat alpha-lactalbumin was prepared from whey by chromatography on DEAE-Sephadex A-50 and Ultrogel AcA 44. Two biologically active forms of alpha-lactalbumin were apparent after ion-exchange chromatography, but on gel filtration the combined forms were eluted as a single peak with a molecular weight of approx. 33000. The molecular weight when determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis was 15100. Antiserum to alpha-lactalbumin was prepared from rabbits, and single radial immunodiffusion was used to measure the concentration of alpha-lactalbumin in milk expressed from rats during lactation and for 2 days after the cessation of lactation. A significant positive correlation (r = + 0.89) between the concentrations of alpha-lactalbumin and lactose was obtained for the first 20 days of lactation. This is consistent with the suggestion that alpha-lactalbumin may control the concentration of lactose in milk. However, a significant negative correlation (r = -0.91) between the concentration of alpha-lactalbumin and lactose was obtained for 2 days after the cessation of lactation on day 20.

History

Journal

The Biochemical journal

Volume

194

Pagination

149-154

Location

England

ISSN

0264-6021

eISSN

1470-8728

Language

en

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

1

Publisher

Portland Press Ltd.

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