File(s) under permanent embargo
An acetylcholinesterase inhibition biosensor based on a reduced graphene oxide/silver nanocluster/chitosan nanocomposite for detection of organophosphorus pesticides
journal contribution
posted on 2015-01-01, 00:00 authored by Y Zhang, H Liu, Z Yang, S Ji, J Wang, P Pang, L Feng, H Wang, Z Wu, Wenrong YangWenrong YangA sensitive electrochemical acetylcholinesterase (AChE) biosensor based on a reduced graphene oxide (rGO) and silver nanocluster (AgNC) modified glassy carbon electrode (GCE) was developed. rGO and AgNC nanomaterials with excellent conductivity, catalytic activity and biocompatibility offered an extremely hydrophilic surface, which facilitated the immobilization of AChE to fabricate the organophosphorus pesticide biosensor. Carboxylic chitosan (CChit) was used as a cross-linker to immobilize AChE on a rGO and AgNC modified GCE. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl. Based on the inhibition effect of organophosphorus pesticides on the AChE activity, using phoxim as a model compound, the inhibition effect of phoxim was proportional to its concentration ranging from 0.2 to 250 nM with a detection limit of 81 pM estimated at a signal-to-noise ratio of 3. The developed biosensor exhibited good sensitivity, stability and reproducibility, thus providing a promising tool for analysis of enzyme inhibitors and direct analysis of practical samples.
History
Journal
Analytical methodsVolume
7Issue
15Pagination
6213 - 6219Publisher
Royal Society of ChemistryLocation
London, Eng.Publisher DOI
ISSN
1759-9660eISSN
1759-9679Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, Royal Society of ChemistryUsage metrics
Categories
No categories selectedKeywords
Science & TechnologyPhysical SciencesLife Sciences & BiomedicineTechnologyChemistry, AnalyticalFood Science & TechnologySpectroscopyChemistrySURFACE-PLASMON RESONANCESILVER NANOCLUSTERSMODIFIED ELECTRODEAMPEROMETRIC BIOSENSORLIQUID-CHROMATOGRAPHYSENSITIVE DETECTIONCARBON ELECTRODENANOPARTICLESOXIDEPHOXIM
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC