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An indirect enzyme-linked immunosorbent assay for measurement of abscisic acid in soybean inoculated with Phytophthora megasperma f. sp. glycinea

journal contribution
posted on 1989-11-01, 00:00 authored by David CahillDavid Cahill, E W B Ward
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to measure the concentration of abscisic acid (ABA) in soybean hypocotyl tissues after inoculation with Phytophthora megasperma f. sp. glycinea. The assay, which uses monoclonal antibodies, has a measuring range of 5-250 pg of ABA. Validation of the assay with high-performance liquid chromatography showed that the assay is a sensitive and reliable method for the determination of ABA in crude tissue extracts. In an incompatible interaction the concentration of ABA in lesions was reduced to 32% of uninoculated control levels by 6 hr after inoculation.

History

Journal

Phytopathology

Volume

79

Issue

11

Pagination

1238 - 1242

Publisher

American Phytopathological Society

Location

St. Paul, MN

ISSN

0031-949X

Publication classification

CN.1 Other journal article

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