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Analysis of self-assembled monolayer interfaces by electrospray mass spectrometry : a gentle approach

journal contribution
posted on 2003-01-01, 00:00 authored by Wenrong YangWenrong Yang, R Zhang, G Willett, D Hibbert, J Gooding
A general mass spectrometry technique for the characterization of alkanethiol-modified surfaces is presented. Alkanethiol self-assembled onto a gold surface (in this case, peptides were attached to the gold surface via a thiolate bond) was reductively desorbed in 0.05 M KOH in the presence of octadecyl-derivatized silica gel. The peptide adsorbed onto the silica gel, whereupon it could be filtered, washed to remove any salts, and then eluted using a mixture of 4:1 v/v methanol/water. The eluant containing the peptide was injected into a Fourier transform ion-cyclotron resonance mass spectrometer (FTICR/MS) via electrospray ionization. The spectrum showed no fragmentation of the peptide, demonstrating the gentleness of the technique. This simple procedure is not limited to FTICR/MS and could be adapted to other mass spectrometers.

History

Journal

Analytical chemistry

Volume

75

Issue

23

Pagination

6741 - 6744

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

0003-2700

eISSN

1520-6882

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2003, American Chemical Society

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