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Anthrones, naturally occurring competitive inhibitors of adenosine‐triphosphate‐citrate lyase
journal contribution
posted on 1995-09-01, 00:00 authored by J J Oleynek, Colin BarrowColin Barrow, M P Burns, D M Sedlock, D J Murphy, P V Kaplita, H H Sun, R Cooper, A M Gillum, C C ChadwickAdenosine triphosphate (ATP)‐citrate lyase (ACL) is a homotetrameric liver enzyme which catalyzes the cytosolic formation of acetyl‐CoA. This reaction provides the major source of acetyl‐CoA for fatty acid and cholesterol biosynthesis. Inhibition of ACL offers a potentially unique way to control plasma cholesterol and triglyceride levels. In this study, we describe the activity of an active microbial metabolite derived from a soil fungus, Penicillium sp., SC2193. This culture produces a series of related anthrones and anthraquinones. We present evidence for one of these anthrones, 2‐chloro‐1, 3, 8‐trihydroxy‐6‐methyl‐9‐anthrone, as a specific and competitive inhibitor of ACL against the substrate Mg citrate and mixed noncompetitive inhibition against two other required substrates for this enzyme, Mg ATP and CoA. With an IC 50 of 283 nM in the primary assay and, more specifically, a K i of < 100 nM against the substrate Mg citrate, SC2193 represents the most potent competitive inhibitor of ACL yet described, and, as such, might prove very useful as a molecular tool for the discovery of selective, mechanistically novel hypolipidemic agents.
History
Journal
Drug development researchVolume
36Issue
1Pagination
35 - 42Publisher
Wiley-LissLocation
Hoboken, N.J.Publisher DOI
ISSN
0272-4391eISSN
1098-2299Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1995, Wiley-Liss, Inc.Usage metrics
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No categories selectedKeywords
Adenosine triphosphate (ATP)-citrate lyase (ACL)homotetrameric liver enzymecytosolic formationacetyl-CoAfatty acidcholesterol biosynthesisplasma cholesteroltriglyceridesoil fungusPenicillium sp., SC2193anthronesanthraquinonesanthrones, 2-chloro-1, 3, 8-trihydroxy-6-methyl-9-anthroneMg ATPCoAhypolipidemic agents
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