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Antibodies against merozoite surface protein (MSP)-1(19) are a major component of the invasion-inhibitory response in individuals immune to malaria

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posted on 2024-06-03, 12:27 authored by RA O'Donnell, Tania De Koning-WardTania De Koning-Ward, RA Burt, M Bockarie, JC Reeder, AF Cowman, BS Crabb
Antibodies that bind to antigens expressed on the merozoite form of the malaria parasite can inhibit parasite growth by preventing merozoite invasion of red blood cells. Inhibitory antibodies are found in the sera of malaria-immune individuals, however, the specificity of those that are important to this process is not known. In this paper, we have used allelic replacement to construct a Plasmodium falciparum parasite line that expresses the complete COOH-terminal fragment of merozoite surface protein (MSP)-1(19) from the divergent rodent malaria P. chabaudi. By comparing this transfected line with parental parasites that differ only in MSP-1(19), we show that antibodies specific for this domain are a major component of the inhibitory response in P. falciparum-immune humans and P. chabaudi-immune mice. In some individual human sera, MSP-1(19) antibodies dominated the inhibitory activity. The finding that antibodies to a small region of a single protein play a major role in this process has important implications for malaria immunity and is strongly supportive of further understanding and development of MSP-1(19)-based vaccines.

History

Journal

Journal of experimental medicine

Volume

193

Pagination

1403-1412

Location

New York, N.Y.

ISSN

0022-1007

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2001, Rockefeller University Press

Issue

12

Publisher

Rockefeller University Press