stevanovic-applicationofa-2019.pdf (1.77 MB)
Application of a fluorescent probe for the online measurement of PM-bound reactive oxygen species in chamber and ambient studies
journal contribution
posted on 2019-01-01, 00:00 authored by Reece Brown, Svetlana StevanovicSvetlana Stevanovic, Z Brown, M Cai, S Zhou, W Song, X Wang, B Miljevic, J Zhao, S Bottle, Z RistovskiThis manuscript details the application of a profluorescent nitroxide (PFN) for the online quantification of radical concentrations on particulate matter (PM) using an improved Particle Into Nitroxide Quencher (PINQ). A miniature flow-through fluorimeter developed specifically for use with the 9,10-bis(phenylethynyl)anthracene-nitroxide (BPEAnit) probe was integrated into the PINQ, along with automated gas phase corrections through periodic high efficiency particle arrestor (HEPA) filtering. The resulting instrument is capable of unattended sampling and was operated with a minimum time resolution of 2.5 min. Details of the fluorimeter design and examples of data processing are provided, and results from a chamber study of side-stream cigarette smoke and ambient monitoring campaign in Guangzhou, China are presented. Primary cigarette smoke was shown to have both short-lived (t1/2 = 27 min) and long-lived (t1/2 = indefinite) PM-bound reactive oxygen species (ROS) components which had previously only been observed in secondary organic aerosol (SOA).
History
Journal
SensorsVolume
19Issue
20Article number
4564Pagination
1 - 16Publisher
MDPILocation
Basel, SwitzerlandPublisher DOI
Link to full text
ISSN
1424-8220Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalUsage metrics
Categories
Keywords
Oxidative stressReactive oxygen speciesProfluorescent nitroxideParticulate matterAerosolBPEAnitScience & TechnologyPhysical SciencesTechnologyChemistry, AnalyticalEngineering, Electrical & ElectronicInstruments & InstrumentationChemistryEngineeringDITHIOTHREITOL DTT ASSAYAIR-POLLUTIONFREE-RADICALSROSMONITORHEALTHQUANTIFICATIONINSTRUMENTPARTICLESDistributed ComputingEcology
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC