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Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography
journal contribution
posted on 2015-05-01, 00:00 authored by Danielle Bassanese, Xavier ConlanXavier Conlan, Neil BarnettNeil Barnett, Paul StevensonThis paper explores the analytical figures of merit of two-dimensional high-performance liquid chromatography for the separation of antioxidant standards. The cumulative two-dimensional high-performance liquid chromatography peak area was calculated for 11 antioxidants by two different methods--the areas reported by the control software and by fitting the data with a Gaussian model; these methods were evaluated for precision and sensitivity. Both methods demonstrated excellent precision in regards to retention time in the second dimension (%RSD below 1.16%) and cumulative second dimension peak area (%RSD below 3.73% from the instrument software and 5.87% for the Gaussian method). Combining areas reported by the high-performance liquid chromatographic control software displayed superior limits of detection, in the order of 1 × 10(-6) M, almost an order of magnitude lower than the Gaussian method for some analytes. The introduction of the countergradient eliminated the strong solvent mismatch between dimensions, leading to a much improved peak shape and better detection limits for quantification.
History
Journal
Journal separation scienceVolume
38Issue
10Pagination
1642 - 1648Publisher
WileyLocation
London, Eng.Publisher DOI
eISSN
1615-9314Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, WileyUsage metrics
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No categories selectedKeywords
AntioxidantsCountergradientLimit of detectionSolvent strength mismatchTwo-dimensional high-performance liquid chromatographyScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryTANDEM MASS-SPECTROMETRYDIODE-ARRAY DATASTEVIA-REBAUDIANAMOBILE-PHASEQUANTIFICATIONPROTEOMICSINJECTIONPEAKS2ND-DIMENSIONCOLUMNS
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