File(s) under permanent embargo
Augmented platelet calcium uptake in response to serotonin stimulation in patients with major depression measured using Mn2+ influx and 45Ca2+ uptake.
journal contributionposted on 2000-01-01, 00:00 authored by H Plein, Michael BerkMichael Berk, S Eppel, N Butkow
There is an augmented platelet intracellular calcium response to serotonin stimulation in major depression. The role that calcium influx has in this process is not known. The objective of this study was to determine platelet calcium influx in response to serotonin by two methods, Mn2+ influx and 45Ca2+ uptake, in order to observe if the uptake response to serotonin was augmented in major depression by comparing the response to normal controls. The use of the two methods of calcium influx showed that serotonin stimulates calcium uptake into platelets. Furthermore, patients with major depression have significantly augmented platelet calcium uptake in response to serotonin. The interesting finding was that calcium uptake into platelets is biphasic, occurring immediately and after five minutes. These results may support the two pool model for calcium oscillations within cells whereby extracellular calcium is needed for intracellular calcium release, and for replenishment of depleted stores once intracellular calcium is released.
Pagination425 - 431
Publication classificationC1.1 Refereed article in a scholarly journal
Copyright notice2000, Elsevier
AdultAgedBlood PlateletsCalciumCalcium RadioisotopesCations, DivalentDepressive DisorderDose-Response Relationship, DrugFemaleFluorescenceFura-2HumansIon TransportKineticsMaleManganeseMiddle AgedSerotoninScience & TechnologyLife Sciences & BiomedicineMedicine, Research & ExperimentalPharmacology & PharmacyResearch & Experimental MedicineplateletCa-45(2+) uptakemajor depressionintracellular Mn2+ influxPLASMA-MEMBRANECA-2+ INFLUXDISORDERSMODEL