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Biocompatibility and modification of the protein-based adhesive secreted by the Australian frog Notaden bennetti
journal contribution
posted on 2010-05-01, 00:00 authored by L D Graham, S J Danon, G Johnson, C Braybrook, N K Hart, Russell VarleyRussell Varley, M D M Evans, G A McFarland, M J Tyler, J A Werkmeister, J A M RamshawWhen provoked, Notaden bennetti frogs secrete a proteinaceous exudate, which rapidly forms a tacky and elastic glue. This material has potential in biomedical applications. Cultured cells attached and proliferated well on glue-coated tissue culture polystyrene, but migrated somewhat slower than on uncoated surfaces. In organ culture, dissolved glue successfully adhered collagen-coated perfluoropolyether lenses to debrided bovine corneas and supported epithelial regrowth. Small pellets of glue implanted subcutaneously into mice were resorbed by surrounding tissues, and all of the animals made a full recovery. An initial but transient skin necrosis at the implant site was probably caused by some of the potentially toxic metabolites present in the frog secretion; these include sterols and carotenoids, as well as fatty alcohols, aldehydes, ketones, acids, and aromatic compounds. Removal of the carotenoid pigments did not significantly alter the glue's material properties. In contrast, peroxidase treatment of dissolved glue introduced unnatural crosslinks between molecules of the major protein (Nb-1R) and resulted in the formation of a soft hydrogel, which was very different to the original material.
History
Journal
Journal of Biomedical Materials Research Part AVolume
93Issue
2Pagination
429 - 441Publisher
WileyLocation
United StatesPublisher DOI
ISSN
1549-3296eISSN
1552-4965Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2009 Wiley PeriodicalsUsage metrics
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No categories selectedKeywords
AcetoneAdhesivesAnimalsAnuraBiocompatible MaterialsCattleCell AdhesionCell Culture TechniquesCell MovementCells, CulturedCorneaEndotoxinsFemaleGas Chromatography-Mass SpectrometryMaterials TestingMiceMice, Inbred BALB CSolventsSurface PropertiesTissue Culture TechniquesScience & TechnologyTechnologyEngineering, BiomedicalMaterials Science, BiomaterialsEngineeringMaterials ScienceNotadenadhesiveresorptionbiocompatibilitycrosslinkfrog glueBETA-CAROTENECELLSATTACHMENTAPOPTOSISTOXICITYCULTUREBONEGEL
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