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Bioethanol production potential of a novel thermophilic isolate Thermoanaerobacter sp. DBT-IOC-X2 isolated from Chumathang hot spring
journal contributionposted on 2018-09-01, 00:00 authored by Nisha Singh, Munish PuriMunish Puri, D K Tuli, R P Gupta, Colin BarrowColin Barrow, A S Mathur
Dilute acid pretreatment of biomass generates enormous amount of hydrolysate (rich in inhibitors and pentose sugars), that remains unutilized for bioethanol production due to inadequacy of efficient C5-fermenting organisms. In this study, a predominantly pentose fermenting extremely thermophilic bacterium strain DBT-IOC-X2, pertaining to the genus Thermoanaerobacter was isolated from Himalayan hot spring. Batch experiments indicated substantial inhibitor resistance (2 g dm−3for furfural, 5-HMF, and acetic acid), substrate tolerance (∼15 g dm−3), co-sugar fermentation ability (co-production ethanol yield of 0.29 g/g), and high ethanol yield (83.57% and 91.12% of the theoretical maximum from 5 g dm−3glucose and xylose, respectively) by the bacterium at 70 °C and pH 8.0. Here, bioethanol production process was developed using pre-treated rice straw hydrolysate (PRSH) as low-cost agro-waste and 83.47% of the total sugar conversion was obtained. This study shows that Thermoanaerobacter sp. DBT-IOC-X2 could utilize diluted PRSH efficiently to improve the overall cost-effectiveness of biomass processing to bioethanol.
JournalBiomass and bioenergy
Pagination122 - 130
LocationAmsterdam, The Netherlands
Publication classificationC Journal article; C1 Refereed article in a scholarly journal
Copyright notice2018, Elsevier Ltd.
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ThermoanaerobacterPentose-rich sugar hydrolysateBioethanolHot springRice strawScience & TechnologyLife Sciences & BiomedicineTechnologyAgricultural EngineeringBiotechnology & Applied MicrobiologyEnergy & FuelsAgricultureETHANOL-PRODUCTIONCLOSTRIDIUM-THERMOHYDROSULFURICUMANAEROBIC BACTERIUMHYDROGEN-PRODUCTIONWHEAT-STRAWGEN-NOVFERMENTATIONXYLOSEMECHANISMS