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Block copolymer conjugates prepared by sequentially grafting from proteins via RAFT

Version 2 2024-06-13, 12:17
Version 1 2018-06-19, 10:47
journal contribution
posted on 2024-06-13, 12:17 authored by Hongmei Li, Ming Li, Xiao Yu, Abhijeet P Bapat, Brent S Sumerlin
Well-defined thermoresponsive block copolymer conjugates were prepared by two consecutive grafting-from polymerizationsvia reversible addition–fragmentation chain transfer (RAFT) polymerization. A RAFT agent containing an activated ester was conjugated to amines on lysozyme, and N-isopropylacrylamide was polymerized by grafting directly from the modified protein in aqueous buffer. Retention of the active thiocarbonylthio moieties on the ω end of the conjugated chains allowed chain extension viapolymerization of N,N-dimethylacrylamide to yield lysozyme–poly(N-isopropylacrylamide)-b-poly(N,N-dimethylacrylamide) (LYS–PNIPAM-b-PDMA) block copolymer conjugates. Analysis of the conjugates, as well as the cleaved homopolymers and block copolymers, indicated the grafting-from polymerizations were well controlled and that chain extension during block copolymerization was efficient. The temperature-responsive aqueous solution behavior of the resulting conjugates was investigated.

History

Journal

Polymer chemistry

Volume

2

Pagination

1531-1535

Location

Cambridge, Eng.

ISSN

1759-9954

eISSN

1759-9962

Language

eng

Publication classification

CN.1 Other journal article

Copyright notice

2011, The Royal Society of Chemistry

Issue

7

Publisher

Royal Society of Chemistry