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COIMMUNOLOCALIZATION OF TOPOISOMERASE-II AND CHLOROPLAST DNA IN DEVELOPING, DIVIDING AND MATURE WHEAT CHLOROPLASTS

journal contribution
posted on 2023-11-02, 04:54 authored by JL MARRISON, Rebecca LeechRebecca Leech
This paper describes the first localization of immunofluorescence of topoisomerase II in developing chloroplasts. In order to investigate the relationship between topoisomerase II and chloroplast DNA (ctDNA) replication during chloroplast development the 7‐day‐old wheat leaf was used. Topoisomerase II was immunolabelled and fluorescein tagged and the ctDNA simultaneously stained with 4,6‐diamidino‐2‐phenylindole (DAPI) in the same sections. Topoisomerase II was detected at every stage of chloroplast development and maximal levels of topoisomerase II were found in chloroplasts at the time of ctDNA replication. Topoisomerase II was localized around the plastid periphery, exactly mirroring the position of the ctDNA. After chloroplast division both topoisomerase II and ctDNA are seen to be restricted to small discrete areas within the plastid, but at different sites. These findings strongly suggest a role for topoisomerase II in ctDNA decatenation prior to chloroplast division.

History

Journal

PLANT JOURNAL

Volume

2

Pagination

783-790

ISSN

0960-7412

eISSN

1365-313X

Language

English

Issue

5

Publisher

BLACKWELL SCIENCE LTD

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