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Centromeric chromatin pliability and memory at a human neocentromere
journal contribution
posted on 2003-01-01, 00:00 authored by Jeffrey CraigJeffrey Craig, Lee H Wong, Anthony W I Lo, Elizabeth Earle, K H Andy ChooWe show that Trichostatin A (TSA)-induced partial histone hyperacetylation causes a unidirectional shift in the position of a previously defined binding domain for the centromere-specific histone H3 homologue CENP-A at a human neocentromere. The shift of approximately 320 kb is fully reversible when TSA is removed, but is accompanied by an apparent reduction in the density of CENP-A per unit length of genomic DNA at the neocentromere. TSA treatment also instigates a reversible abolition of a previously defined major domain of differentially delayed replication timing that was originally established at the neocentromeric site. None of these changes has any measurable deleterious effects on mitosis or neocentromere function. The data suggest pliability of centromeric chromatin in response to epigenetic triggers, and the non-essential nature of the regions of delayed replication for centromere function. Reversibility of the CENP-A-binding position and the predominant region of delayed replication timing following removal of TSA suggest strong memory at the original site of neocentromeric chromatin formation.
History
Journal
EMBO journalVolume
22Issue
10Pagination
2495 - 2504Publisher
European Molecular Biology OrganizationLocation
Oxford, Eng.Publisher DOI
ISSN
0261-4189Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2003, European Molecular Biology OrganizationUsage metrics
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AcetylationAnimalsAutoantigensCell CycleCell LineCentromereCentromere Protein AChromatinChromosomal Proteins, Non-HistoneChromosomes, Human, Pair 10DNA ReplicationEnzyme InhibitorsHistone Deacetylase InhibitorsHistonesHumansHydroxamic AcidsProtein BindingCENP‐ANeocentromereReplication TimeScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyCell BiologyCENP-AHISTONE ACETYLATIONALPHA-SATELLITECYTOGENETIC ANALYSISDNA-REPLICATIONCHROMOSOMEPROTEINHETEROCHROMATINORGANIZATIONMETHYLATION
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