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Characterization of Early Pathogenic Effects after Experimental Infection of Calves with Bovine Immunodeficiency-Like Virus.
journal contributionposted on 1992-02-01, 00:00 authored by S Carpenter, L D Miller, Soren AlexandersenSoren Alexandersen, C A Whetstone, M J VanDerMaaten, B Viuff, Y Wannemuehler, J M Miller, J A Roth
The early pathogenic effects of bovine immunodeficiency-like virus (BIV) were studied in calves experimentally inoculated with BIV. All animals inoculated with BIV R29-infected cells seroconverted by 6 weeks postinoculation, and BIV was recoverable from each animal at 2 weeks postinoculation. However, levels of BIV replication in vivo appeared to be low. In situ hybridization studies indicated that during peak periods of viral replication in vivo, less than 0.03% of peripheral blood mononuclear cells were expressing detectable levels of viral RNA. Moreover, the levels of viral RNA in these cells in vivo were less than 1/10 the levels observed in persistently infected cells in vitro. BIV-inoculated calves had significantly higher numbers of circulating lymphocytes, and follicular hyperplasia was observed in lymph nodes, hemal nodes, and spleen. The histopathological changes observed in BIV-infected calves were similar to changes found early after infection with the immunosuppressive lentiviruses, including human immunodeficiency virus type 1.
JournalJournal of Virology
Pagination1074 - 1083
PublisherAmerican Society for Microbiology
Publication classificationCN.1 Other journal article
CategoriesNo categories selected
AnimalsAntibodies, ViralCattleCattle DiseasesImmunodeficiency Virus, BovineLentivirus InfectionsLeukocyte CountLymph NodesLymphocytesNeutrophilsRepetitive Sequences, Nucleic AcidTime FactorsScience & TechnologyLife Sciences & BiomedicineVirologyMINK DISEASE PARVOVIRUSBIOLOGICALLY-ACTIVE PROVIRUSESLYMPH-NODE HISTOLOGYNUCLEOTIDE-SEQUENCEANEMIA VIRUSHYBRIDIZATION PROBESINSITU HYBRIDIZATIONVIRAL REPLICATIONMOLECULAR-CLONINGALEUTIAN DISEASE