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Characterization of variable regions in the envelope and S3 open reading frame of equine infectious anemia virus

journal contribution
posted on 1991-08-01, 00:00 authored by Soren AlexandersenSoren Alexandersen, S Carpenter
The polymerase chain reaction was used to amplify and clone parts of the envelope gene and overlapping S3 open reading frame, thought to encode rev, of the virulent in vivo-derived Th-1 isolate of equine infectious anemia virus (EIAV). The results indicated that EIAV consists of a heterogeneous mixture of genotypes present at the first febrile cycle after initial infection. We showed that the Th-1 isolate apparently contains nondefective genotypes as well as types which have transmembrane protein truncations or are rev deficient. Furthermore, we could confirm the presence of a hypervariable region in the gp90 envelope glycoprotein. Taken together with earlier data on the heterogeneity of the regulatory motifs present in the long terminal repeat sequences of viruses from the same in vivo isolate (S. Carpenter, S. Alexandersen, M. J. Long, S. Perryman, and B. Chesebro, J. Virol. 65:1605-1610, 1991), our findings indicate that EIAV uses a complex system of diversity in biological phenotypes together with variation in regulatory and antigenic makeup to evade host response and to cause persistent infection and recurrent chronic disease.

History

Journal

Journal of virology

Volume

65

Issue

8

Pagination

4255 - 4262

Publisher

American Society for Microbiology

Location

United States

ISSN

0022-538X

Language

eng

Publication classification

CN.1 Other journal article