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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review

Version 3 2024-06-19, 22:48
Version 2 2024-06-03, 02:01
Version 1 2023-11-29, 04:28
journal contribution
posted on 2024-06-19, 22:48 authored by Soomin LeeSoomin Lee, HK Balakrishnan, Egan DoevenEgan Doeven, D Yuan, Rosanne GuijtRosanne Guijt
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological threats, including infectious diseases. Prior to nucleic acid amplification and detection, extensive sample preparation techniques are required to free nucleic acids and extract them from the sample matrix. Sample preparation is critical to maximize the sensitivity and reliability of testing. As the enzymatic amplification reactions can be sensitive to inhibitors from the sample, as well as from chemicals used for lysis and extraction, avoiding inhibition is a significant challenge, particularly when minimising liquid handling steps is also desirable for the translation of the assay to a portable format for PONT. The reagents used in sample preparation for nucleic acid testing, covering lysis and NA extraction (binding, washing, and elution), are reviewed with a focus on their suitability for use in PONT.

History

Journal

Biosensors

Volume

13

Article number

980

Pagination

980-980

Location

Switzerland

ISSN

2079-6374

eISSN

2079-6374

Language

en

Publication classification

C1 Refereed article in a scholarly journal

Issue

11

Publisher

MDPI AG