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Chemiluminescence detection flow cells for flow injection analysis and high-performance liquid chromatography
journal contribution
posted on 2012-06-01, 00:00 authored by Jessica Terry, S Mohr, P Fielden, N Goddard, Neil BarnettNeil Barnett, D Olson, D Wolcott, Paul FrancisPaul FrancisWe have examined a range of new and previously described flow cells for chemiluminescence detection. The reactions of acidic potassium permanganate with morphine and amoxicillin were used as model systems representing the many fast chemiluminescence reactions between oxidising agents and organic analytes, and the preliminary partial reduction of the reagent was exploited to further increase the rates of reaction. The comparison was then extended to high-performance liquid chromatography separations of α- and β-adrenergic agonists, with permanganate chemiluminescence detection. Flow cells constructed by machining novel channel designs into white polymer materials (sealed with transparent films or plates) have enabled improvements in mixing efficiency and overall transmission of light to the photodetector.
History
Journal
Analytical and bioanalytical chemistryVolume
403Issue
8Pagination
2353 - 2360Publisher
SpringerLocation
Heidelberg, GermanyISSN
1618-2642eISSN
1618-2650Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2012, Springer-VerlagUsage metrics
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No categories selectedKeywords
acidic potassium permanganatechemiluminescenceflow cellshigh-performance liquid chromatographyScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryACIDIC POTASSIUM-PERMANGANATESOLID-PHASE EXTRACTIONSYSTEMANTIOXIDANTSSALBUTAMOLSYNEPHRINEALKALOIDSCAPACITYCHIPHPLC
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