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Chemiluminescence from the oxidation of urea and ammonia with hypobromite and N-bromosuccinimide
journal contribution
posted on 2004-10-08, 00:00 authored by Paul FrancisPaul Francis, Neil BarnettNeil Barnett, S Lewis, Kieran LimKieran LimThe spectral distribution for the chemiluminescent oxidation of ammonia with hypobromite is significantly different to that for the oxidation of ammonia with N-bromosuccinimide. Therefore, in contrast to the assumptions of several authors, the action of N-bromosuccinimide is not solely derived from the in situ formation of hypobromite. Neither the oxidation of urea with hypobromite nor the oxidation of urea with N-bromosuccinimide involves an initial hydrolysis of urea to ammonia in the alkaline solution. However, these two reactions lead to a common emitter. The addition of xanthene dyes, such as dichlorofluorescein, enhance the chemiluminescence intensity by energy transfer to the efficient fluorophore, but reaction between the sensitiser and hypobromite can result in a significant increase in the background signal. A list of potential interferences has been compiled; particular attention was paid to guanidino compounds, as the chemiluminescence accompanying the oxidation of this functional group has not been previously discussed.
History
Journal
TalantaVolume
64Issue
2Pagination
283 - 289Publisher
Elsevier B.V.Location
Amsterdam, NetherlandsPublisher DOI
ISSN
0039-9140eISSN
1873-3573Language
engNotes
Available online 19 May 2004.Publication classification
C1 Refereed article in a scholarly journalCopyright notice
2004, Elsevier B.V.Usage metrics
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