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Chicken anemia virus : an understanding of the in-vitro host response over time
journal contribution
posted on 2011-02-01, 00:00 authored by Tamsyn CrowleyTamsyn Crowley, V Haring, R MooreChicken anemia virus (CAV) is an economically important virus affecting the chicken meat and egg industry. CAV is characterized by anemia, lymphoid depletion, and immunosuppression. Microarrays were used to investigate the response of MDCC-MSB1 cells (MSB1) to infection with CAV at 24 and 48 h post-infection (hpi). The major genes responding to CAV infection include genes involved in inflammation, apoptosis, and antiviral activity. Several cytokines were differentially regulated at either 24 or 48 hpi, including interleukin 2 (IL-2), interleukin receptors IL-1R, IL-22R, IL-18R, and IL-7R, and interferon-α (IFN-α). While there were many genes differentially regulated in this experiment, only two genes were common to both time points, suggesting a dramatic change in gene expression over the two time points studied. The present study is the first microarray experiment to investigate CAV, and we identified a number of key pathways involved in viral infection. Overall, there were more genes upregulated at 24 hpi than at 48 hpi, including genes involved in cytokine signaling, apoptosis, and antiviral activity. The two time points were vastly different in their gene expression patterns, in that at 24 hpi there were many genes involved in the response to infection, whereas at 48 hpi there were many genes associated with apoptosis and immunosuppression.
History
Journal
Viral immunologyVolume
24Issue
1Pagination
3 - 9Publisher
Mary Ann Liebert PublishersLocation
New Rochelle, N. Y.Publisher DOI
ISSN
0882-8245eISSN
1557-8976Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2011, Mary Ann Liebert, Inc.Usage metrics
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Keywords
animalscell Linechicken anemia viruschickenscytokinesgene Expression Profilinghost-Pathogen Interactionsmicroarray Analysistime FactorsScience & TechnologyLife Sciences & BiomedicineImmunologyVirologyMAREKS-DISEASE VIRUSMAJOR HISTOCOMPATIBILITY COMPLEXGENE-EXPRESSIONTRANSCRIPTIONAL ANALYSISPHYLOGENETIC ANALYSISMOLECULAR-MECHANISMSVIRAL-INFECTIONCDNA CLONINGPROTEINAPOPTOSISImmunology
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