Chromatographic Evidence for the Presence of Multiple Tachykinins in the Bovine Adrenal Medulla.
Version 2 2024-06-18, 01:29Version 2 2024-06-18, 01:29
Version 1 2017-08-03, 11:57Version 1 2017-08-03, 11:57
journal contribution
posted on 2024-06-18, 01:29authored byS Basile, NS Cheung, BG Livett
Among the mammalian tachykinins, substance P (SP) has been shown to be the most potent at modulating the response due to nicotinic acetylcholine receptor stimulation of bovine adrenal chromaffin cells. SP-like immunoreactivity has been detected in nerve terminals innervating the adrenal medulla; however, little is known of the presence of other tachykinins in this tissue. In this study, reverse-phase HPLC was used to fractionate peptides in bovine adrenal medullary extracts, and the fractions were analyzed by radioimmunoassay using antisera to SP or neurokinin A (NKA). The results show that both NKA- and SP-like immunoreactivities are present in the adrenal medulla. The presence of neurokinin B is also indicated. The presence of multiple tachykinins in this tissue raises questions as to their functions in the adrenal medulla.