Chronic predator stress in female mice reduces primordial follicle numbers: implications for the role of ghrelin
journal contributionposted on 2019-01-01, 00:00 authored by M R Di Natale, A Soch, I Ziko, S N De Luca, S J Spencer, Luba SominskyLuba Sominsky
Chronic stress is a known suppressor of female reproductive function. However, attempts to isolate single causal links between stress and reproductive dysfunction have not yet been successful due to their multi-faceted aetiologies. The gut-derived hormone ghrelin regulates stress and reproductive function and may therefore be pivotal in the neuroendocrine integration of the hypothalamic–pituitary–adrenal (HPA) and –gonadal (HPG) axes. Here, we hypothesised that chronic stress disrupts ovarian follicle maturation and that this effect is mediated by a stress-induced increase in acyl ghrelin and activation of the growth hormone secretatogue receptor (GHSR). We gave C57BL/6J female mice 30 min daily chronic predator stress for 4 weeks, or no stress, and gave them daily GHSR antagonist (d-Lys3-GHRP-6) or saline. Exposure to chronic predator stress reduced circulating corticosterone, elevated acyl ghrelin levels and led to significantly depleted primordial follicle numbers. GHSR antagonism stress-dependently altered the expression of genes regulating ovarian responsiveness to gonadotropins and was able to attenuate the stress-induced depletion of primordial follicles. These findings suggest that chronic stress-induced elevations of acyl ghrelin may be detrimental for ovarian follicle maturation.
JournalJournal of endocrinology
Pagination201 - 219
Publication classificationC1 Refereed article in a scholarly journal
acyl ghrelinANTI-MULLERIAN HORMONECORTICOTROPIN-RELEASING-FACTOREndocrinology & MetabolismGHSRGRANULOSA-CELLSHORMONE SECRETAGOGUE RECEPTORHUMAN OVARIAN-FOLLICLESINDUCED SUPPRESSIONLife Sciences & BiomedicineLUTEINIZING-HORMONEovaryScience & TechnologySEX-DIFFERENCESSOCIAL TRANSMISSIONSTIMULATING-HORMONEstress