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Citric acid inhibits Cd uptake by improving the preferential transport of Mn and triggering the defense response of amino acids in grains

journal contribution
posted on 2024-03-13, 03:25 authored by Weijie Xue, Peipei WangPeipei Wang, Li Tang, Changbo Zhang, Changrong Wang, Yongchun Huang, Xin Zhang, Yaokui Li, Bingran Zhao, Zhongqi Liu
Citric acid (CA) can regulate the balance of anions and cations in plants, and improve their resistance to heavy metals. It is not clear if foliar application with CA has any effect on migration of Cd and Mn in rice plant. In this work, a low-Cd-accumulating indica rice line (P7) and a high-Cd-accumulating line (HZ) were used to investigate the influence of CA on the transport of Cd and Mn as well as amino acid metabolism in grains. Content of Cd in grains and other organs increased with the increase of Cd content (0.1-2.4 mg kg-1) in soil, while decreased with the foliar application with CA. With the increase of Cd content in rice grains, the content of most amino acids in HZ, P7, HZ+CA and P7 + CA showed an obvious decline trend. Foliar application with CA efficiently raised the Mn:Cd ratio in grains and nodes of both HZ and P7. Meanwhile, the expression levels of OsNramp2, 3 and 5 in panicles were efficiently enhanced by CA application when plants grew in soil with Cd content of 0.6-2.4 mg kg-1. The increasing effect of CA on the content of 4 amino acids (i.e., Glu, Phe, Thr and Ala) in grains was related to varieties and Cd pollution. These results indicate that foliar application with CA can regulate the transport of Cd and Mn in the opposite directions in tissues and inhibit Cd accumulation in grains by enhancing expression of OsNRAMP 2, 3 or 5 and triggering the defense response of some amino acids in Cd-contaminated environment.

History

Journal

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

Volume

211

Article number

ARTN 111921

Location

Netherlands

ISSN

0147-6513

eISSN

1090-2414

Language

English

Publication classification

C1.1 Refereed article in a scholarly journal

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE