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Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor

journal contribution
posted on 2011-01-01, 00:00 authored by P Munish, A Kaur, Colin BarrowColin Barrow, R Singh
Staphylococcus xylosus MAK2, Gram-positive coccus, a nonpathogenic member of the coagulase-negative Staphylococcus family was isolated from soil and used to produce naringinase in a stirred tank reactor. An initial medium at pH 5.5 and a cultivation temperature of 30°C was found to be optimal for enzyme production. The addition of Ca+2 caused stimulation of enzyme activity. The effect of various physico-chemical parameters, such as pH, temperature, agitation, and inducer concentration was studied. The enzyme production was enhanced by the addition of citrus peel powder (CPP) in the optimized medium. A twofold increase in naringinase production was achieved using different technological combinations. The process optimization using technological combinations allowed rapid optimization of large number of variables, which significantly improved enzyme production in a 5-l reactor in 34 h. An increase in sugar concentration (15 gl-1) in the fermentation medium further increased naringinase production (8.9 IUml-1) in the bioreactor. Thus, availability of naringinase renders it attractive for potential biotechnological applications in citrus processing industry.

History

Journal

Applied microbiology and biotechnology

Volume

89

Issue

3

Pagination

715 - 722

Publisher

Springer

Location

Heidelberg, Germany

ISSN

0175-7598

eISSN

1432-0614

Language

eng

Notes

Published online 5 October 2010

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2010, Springer-Verlag