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Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Version 2 2024-06-03, 15:31
Version 1 2016-03-08, 10:40
journal contribution
posted on 2024-06-03, 15:31 authored by NR Berlon, R Qi, BK Sharma-Kuinkel, HS Joo, LP Park, D George, JT Thaden, JA Messina, SA Maskarinec, M Mueller-Premru, Eugene AthanEugene Athan, P Tattevin, JM Pericas, CW Woods, M Otto, VG Fowler
BACKGROUND: Phenol-soluble modulins (PSMs) are amphipathic, pro-inflammatory proteins secreted by most Staphylococcus aureus isolates. This study tested the hypothesis that in vitro PSM production levels are associated with specific clinical phenotypes. METHODS: 177 methicillin-resistant S. aureus (MRSA) isolates from infective endocarditis (IE), skin and soft tissue infection (SSTI), and hospital-acquired/ventilator-associated pneumonia (HAP) were matched by geographic origin, then genotyped using spa-typing. In vitro PSM production was measured by high performance liquid chromatography/mass spectrometry. Statistical analysis was performed using Chi-squared or Kruskal-Wallis tests as appropriate. RESULTS: Spa type 1 was significantly more common in SSTI isolates (62.7% SSTI; 1.7% IE; 16.9% HAP; p < 0.0001) while HAP and IE isolates were more commonly spa type 2 (0% SSTI; 37.3% IE; 40.7% HAP; p < 0.0001). USA300 isolates produced the highest levels of PSMs in vitro. SSTI isolates produced significantly higher quantities of PSMα1-4, PSMβ1, and δ-toxin than other isolates (p < 0.001). These findings persisted when USA300 isolates were excluded from analysis. CONCLUSIONS: Increased in vitro production of PSMs is associated with an SSTI clinical source. This significant association persisted after exclusion of USA300 genotype isolates from analysis, suggesting that PSMs play a particularly important role in the pathogenesis of SSTI as compared to other infection types.

History

Journal

Journal of infection

Volume

71

Pagination

447-457

Location

Amsterdam, The Netherlands

eISSN

1532-2742

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2015, Elsevier

Issue

4

Publisher

Elsevier