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Colorimetric semi-quantitative measurement of pyrophosphate by functionalised SPPS resin in biological media

journal contribution
posted on 2017-05-01, 00:00 authored by Benjamin Long, Fred PfefferFred Pfeffer, Colin BarrowColin Barrow
Inorganic pyrophosphate is a molecule of significant biological importance, which has roles in metabolism and disease diagnosis. In this communication we demonstrate a novel platform for the colorimetric naked eye detection of pyrophosphate using a threshold test in both aqueous solutions and biological fluids. Our system uses peptide synthesis resin as a solid support for a bis-Zn(II)dipicolylamino mediated indicator displacement assay. Our threshold tests are able to detect pyrophosphate in concentrations as low as 1 mM.

History

Journal

Sensors and actuators B: chemical

Volume

243

Pagination

761 - 764

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

0925-4005

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2016, Elsevier