Colorimetric semi-quantitative measurement of pyrophosphate by functionalised SPPS resin in biological media

Inorganic pyrophosphate is a molecule of significant biological importance, which has roles in metabolism and disease diagnosis. In this communication we demonstrate a novel platform for the colorimetric naked eye detection of pyrophosphate using a threshold test in both aqueous solutions and biological fluids. Our system uses peptide synthesis resin as a solid support for a bis-Zn(II)dipicolylamino mediated indicator displacement assay. Our threshold tests are able to detect pyrophosphate in concentrations as low as 1 mM.