shellie-columnselection-2008.pdf (2.49 MB)
Column selection for comprehensive multidimensional ion chromatography
journal contribution
posted on 2008-10-01, 00:00 authored by Robert ShellieRobert Shellie, E Tyrrell, C A Pohl, P R HaddadThis paper discusses the selection of ion chromatography (IC) columns for use in comprehensive multidimensional ion chromatography (IC×IC). First, a single number was determined for a wide range of anions (one number for each anion) using the linear solvent strength model. These numbers were then used to compare the column selectivity characteristics for five different columns. Principal component analysis was used to illustrate selectivity differences between columns. Dionex AS16 and AS20 columns were selected for use in the development of an IC×IC method for the separation of ten anions. To achieve the required speed of analysis in both the first and second separation dimensions, custom column lengths were packed in-house. The use of an eluent suppressor between the first and second columns permits a relatively low flow ratio regime of only <1:20 in the first and second dimensions, respectively, which reduces dilution effects common in comprehensive multidimensional LC. Selection of the second dimension eluent conditions was aided by the development of a spreadsheet based on the linear solvent strength model. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Journal
Journal of Separation ScienceVolume
31Issue
19Pagination
3287 - 3296Publisher
WileyLocation
London, Eng.Publisher DOI
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ISSN
1615-9306eISSN
1615-9314Language
engPublication classification
C1.1 Refereed article in a scholarly journalUsage metrics
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