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Comparative analysis of caveolins in mouse and tammar wallaby: Role in regulating mammary gland function
journal contribution
posted on 2014-11-15, 00:00 authored by S Kuruppath, Julie SharpJulie Sharp, Christophe Lefevre, R M Murphy, Kevin NicholasRecent studies using the mouse showed an inverse correlation between the Caveolin 1 gene expression and lactation, and this was regulated by prolactin. However, current study using mammary explants from pregnant mice showed that while insulin (I), cortisol (F) and prolactin (P) resulted in maximum induction of the β-casein gene, FP and IFP resulted in the downregulation of Caveolin 1. Additionally, IF, FP and IFP resulted in the downregulation of Caveolin 2. Immunohistochemistry confirmed localisation of Caveolin 1 specific to myoepithelial cells and adipocytes. Comparative studies with the tammar wallaby showed Caveolin 1 and 2 had 70-80% homology with the mouse proteins. However, in contrast to the mouse, Caveolin 1 and 2 genes showed a significantly increased level of expression in the mammary gland during lactation. The regulation of tammar Caveolin 1 and 2 gene expression was examined in mammary explants from pregnant tammars, and no significant difference was observed either in the absence or in the presence of IFP.
History
Journal
GeneVolume
552Issue
1Pagination
51 - 58Publisher DOI
ISSN
0378-1119eISSN
1879-0038Publication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2014, ElsevierUsage metrics
Categories
Keywords
Science & TechnologyLife Sciences & BiomedicineGenetics & HeredityCaveolinMouseTammar wallabyMammary glandLactationHormonesCASEIN MESSENGER-RNATRANS-GOLGI-NETWORKMACROPUS-EUGENIIEPITHELIAL-CELLSSCAFFOLDING DOMAINGENE-EXPRESSIONGROWTH-FACTORSIN-VITROPROLACTINPROTEINAdipocytesAmino Acid SequenceAnimalsCaseinsCaveolin 1Caveolin 2Down-RegulationEpithelial CellsFemaleGene ExpressionHydrocortisoneInsulinMacropodidaeMammary Glands, AnimalMiceMice, Inbred BALB CMolecular Sequence DataPregnancySequence Homology, Amino AcidPhysiologyGenetics
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