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Comparative structural analyses of purified glycogen particles from rat liver, human skeletal muscle and commercial preparations
journal contribution
posted on 2009-12-01, 00:00 authored by J H Ryu, Jace Drain, J Kim, Sean McgeeSean Mcgee, A Gray-Weale, L Waddington, G Parker, Mark Hargreaves, S H Yoo, D StapletonGlycogen is a cellular energy store that is crucial for whole body energy metabolism, metabolic regulation and exercise performance. To understand glycogen structure we have purified glycogen particles from rat liver and human skeletal muscle tissues and compared their biophysical properties with those found in commercial glycogen preparations. Ultrastructural analysis of commercial liver glycogens fails to reveal the classical α-rosette structure but small irregularly shaped particles. In contrast, commercial slipper limpet glycogen consists of β-particles with similar branching and chain lengths to purified rat liver glycogen together with a tendency to form small α-particles, and suggest it should be used as a source of glycogen for all future studies requiring a substitute for mammalian liver glycogen.
History
Journal
International journal of biological macromoleculesVolume
45Issue
5Pagination
478 - 482Publisher
Elsevier BVLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
0141-8130eISSN
1879-0003Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2009, Elsevier B.V.Usage metrics
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