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Comparison of cell disruption methods for improving lipid extraction from thraustochytrid strains

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journal contribution
posted on 2015-08-01, 00:00 authored by Avinesh Byreddy, Adarsha Gupta, Colin BarrowColin Barrow, Munish PuriMunish Puri
Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production.

History

Journal

Marine drugs

Volume

13

Issue

8

Pagination

5111 - 5127

Publisher

MDPI

Location

Bern, Switzerland

eISSN

1660-3397

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2015, The Authors