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Complete genome sequence of Rhizobium leguminosarum bv trifolii strain WSM2304, an effective microsymbiont of the South American clover Trifolium polymorphum

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posted on 2010-01-01, 00:00 authored by W Reeve, G O'Hara, P Chain, J Ardley, Lambert BrauLambert Brau, K Nandesena, R Tiwari, S Malfatti, H Kiss, A Lapidus, A Copeland, M Nolan, M Land, N Ivanova, K Mavromatis, V Markowitz, N Kyrpides, V Melino, M Denton, R Yates, J Howieson
Rhizobium leguminosarum bv. trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial Trifolium (clover) species. Strain WSM2304 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from Trifolium polymorphum in Uruguay in 1998. This microsymbiont predominated in the perennial grasslands of Glencoe Research Station, in Uruguay, to competitively nodulate its host, and fix atmospheric nitrogen. Here we describe the basic features of WSM2304, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a nitrogen fixing microsymbiont of a clover species from the American centre of origin. We reveal that its genome size is 6,872,702 bp encoding 6,643 protein-coding genes and 62 RNA only encoding genes. This multipartite genome was found to contain 5 distinct replicons; a chromosome of size 4,537,948 bp and four circular plasmids of size 4,537,948, 1,266,105, 501,946, 308,747 and 257,956 bp.

History

Journal

Standards in genomic sciences

Volume

2

Pagination

66 - 76

Location

East Lansing, Mich.

Open access

  • Yes

ISSN

1944-3277

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, The Authors