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Comprehensive two-dimensional gas chromatography-mass spectrometry and its use in high-resolution metabolomics

journal contribution
posted on 2005-08-31, 00:00 authored by Robert ShellieRobert Shellie
Metabolomics is a comprehensive analysis strategy that aims to identify and quantify the entire suite of metabolites in an organism. The majority of large-scale metabolite profiling studies performed to date[1] have applied multidimensional analysis utilizing chromatography and mass spectrometry (MS). While NMR spectroscopy is an alternative approach for metabolomic analysis,[2] MS-based approaches are generally more sensitive. Moreover, the chromatographic step reduces the number of overlapping signals, so these approaches result in higher numbers of absolute metabolite identifications. Gas chromatography–mass spectrometry (GC–MS) is a tried-and-tested technique for metabolomics;[3,4] however, the quality of the GC–MS information can be improved by using comprehensive two-dimensional GC to enhance the chromatographic resolution.

History

Journal

Australian Journal of Chemistry

Volume

58

Issue

8

Pagination

619 - 619

Publisher

C S I R O Publishing

Location

Clayton, Vic.

ISSN

0004-9425

eISSN

1445-0038

Language

eng

Publication classification

CN.1 Other journal article

Copyright notice

2005, CSIRO

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